Monoclonal Antibody to Cytokeratin 8/18(K8.8 + DC10)
As low as
$499.00
In stock
| Product code: | 36-11110 |
|---|---|
| Clone name : | K8.8 + DC10 |
| Clonality : | Monoclonal |
| Application : | IHC, FACS, WB, IF |
|---|---|
| Reactivity : | Human |
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| Isotype | Mouse IgG1, kappa + Mouse IgG1, kappa |
|---|---|
| Purification | Affinity Chromatography |
| Content | 100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
| Alternative Name | KRT8||CYK8 |
| Gene | KRT8 |
|---|---|
| Gene ID | 3856 |
| Uniprot ID | P05787 |
| Alternative Name | KRT8||CYK8 |
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Application Note:
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA,
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