Monoclonal Antibody to Cytokeratin, pan (Epithelial Marker)(Clone : PAN-CK (Cocktail)
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| Product code: | 36-11035 |
|---|---|
| Clone name : | PAN-CK (Cocktail) |
| Clonality : | Monoclonal |
| Application : | IHC, FACS, WB, IF |
|---|---|
| Reactivity : | Rat |
Shipping Info:
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| Isotype | Mouse IgG, kappa |
|---|---|
| Purification | Affinity Chromatography |
| Content | 100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
| Alternative Name | KRT77||KRT1B |
| Gene | KRT77 |
|---|---|
| Gene ID | 374454 |
| Uniprot ID | Q7Z794 |
| Alternative Name | KRT77||KRT1B |
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 55kDa (CK7); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 46kDa (CK17); 45kDa (CK18) and 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.
Application Note:
Flow Cytometry (0.5-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (0.5-2ug/ml for 2 hours at RT);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections i
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