TNF-alpha Leeporter™ Luciferase Reporter-RAW264.7 Cell Line
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$1,850.00
In stock
| Product code: | 14-106ACL |
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| Application : | Functional Assay |
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Shipping Info:
Order now and get it on Friday April 25, 2025
Same day delivery FREE on San Diego area orders placed by 1.00 PM
| Amount | 1 Vial |
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| Content | Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO. |
| Storage condition | Immediately upon receipt, store in liquid nitrogen. |
The TNF-alpha Leeporterâ„¢ Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-alpha promoter. Tumor necrosis factor-alpha (TNF-alpha) is one of the major proinflammatory cytokines and can induce systemic inflammation, apoptotic cell death, sepsis and cachexia. Dysregulation of TNF-alpha induction is often involved in various human diseases including inflammatory bowel disease, cancer and Alzheimer's disease. The TNF-alpha induction by lipopolysaccharide (LPS), the Toll-like receptor 4 (TLR4) ligand, is shown in Figure 1.
Application Note:
Application:
Monitor the TNF-alpha induction activity.
Screen for activators or inhibitors of the TNF-alpha signaling pathway.
Culture conditions:
Cells should be grown at 37oC with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucos
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